• • Follow procedure for preparation of wash buffer.
• • Check washer alignment daily as part of routine instrument start-up procedures.
• • Ensure that the plate is leveled.
• • Make certain will is completely filled, when washing, to ensure residual conjugate is removed.
• • Examine the fill volume ( a slight dome should be observed at the top of the well).
• • When washing do not allow wells to overflow.
• • Reduce pressure in wash system.
• • Check washers before use to determine they are working properly. Perform routine maintenance.
• • Be certain to wash the specified number of times.
• • Allow approximately 20 seconds soak-time between the addition of wash solution and subsequent aspiration (if soak-time is not indicated in the assay pack insert).
• • Examine the wells for complete aspiration of contents.
• • Upon completion of wash cycle, blot to remove residual fluid.

Learn more: Pipetting tips 丨 Microplate tips 丨 Temperature tips 丨 Incubation tips 丨 Other tips