1798 - First demonstration of vaccination smallpox vaccination (Edward Jenner)

1890 - Demonstration of antibody activity against diphtheria and tetanus toxins. Beginning of humoral theory of immunity. (Emil von Behring) and (Shibasaburo Kitasato)

1900 - Antibody formation theory (Paul Ehrlich)

1938 - Antigen-Antibody binding hypothesis (John Marrack)

1948 - antibody production in plasma B cells

1959-1962 - Discovery of antibody structure

1960 - Radioimmunoassay was first described in a scientific paper by Rosalyn Sussman Yalow and Solomon Berson published in 1960

1966 - A technique to prepare something like immunosorbent to fix antibody or antigen to the surface of a container was published by Wide and Jerker Porath in 1966

1971 - Peter Perlmann and Eva Engvall at Stockholm University invented ELISA

1975 - Generation of the first monoclonal antibodies (George Kohler and Cesar Milstein)