• • Bring microplate pouches to room temperature before opening.
• • Level microwells evenly in microplate frame as the individual breakaway wells have very flexible plate frames leading to bowing off wells and yield poor washes.
• • Place plates in dark immediately after addition of substrate solution, provided the substrate is sensitive to light.
• • Grasp holder on grip marks when tapping to avoid strips slipping from holder.
• • Rotate strips 180 and re-insert or use correct holder if strips do not fit in holder.
• • Seal unused wells in purchase along with the desiccant.
• • Date the pouches when first opened.
• • Clean bottom surface of plates with wash buffer to remove fingerprints.
• • Make sure microwells are at level during washing, reagent addition and plate/strip reading.
• • Wipe the bottom the plate with a lint-free cloth/ towel before reading.
• • Do not allow microwells to become dry once the assay has begun.

Learn more: Washing tips 丨 Pipetting tips 丨 Temperature tips 丨 Incubation tips 丨 Other tips