- • Bring test reagents to room temperature (22-28 C) approximately 30 minutes prior to use.
- • Maintain proper incubation temperature:
- • Lower temperature can decrease OD values.
- • Higher temperatures can increase OD values.
- • Evaporation in wells can cause edging effect.
- • The optimal temperature for incubation is 22-28 C.
- • Check temperature against calibrated thermometer.
- • Strict adherence to time must be maintained.
- • Check calibration of timers.
- • Record time of incubation.
- • Read plate with specified time limits of adding stop solution.
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