• • Calibrate pipettes regularly according to manufacturer's instructions.
• • Avoid touching side wall of well with tips.
• • Avoid splashing of sample and reagents.
• • Avoid blowing out tip contents.
• • Use a new tip for each sample/control/reagent addition.
• • New tips should be used on the multichannel pipettes for each reagent to be added.
• • Reverse pipette when using the multichannel pipettes to add conjugate and substrate solution.
• • Forward pipette when using the multichannel pipettes to add stop solution.
• • Check pipette tips are long enough to provide air space between top of tip and pipette barrel.
• • Check pipette barrel for residual fluid of dried material, remove if present.
• • Ensure pipettes tips are fitted tightly.

Learn more: Washing tips 丨 Microplate tips 丨 Temperature tips 丨 Incubation tips 丨 Other tips